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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: J Cell Biochem. 2009 May 1;107(1):104–111. doi: 10.1002/jcb.22104

Fig. 1.

Fig. 1

IL-12 stimulation and OIP-1 mRNA expression in human bone marrow cells. A: The human bone marrow mononuclear cells were stimulated with IL-12 (0–50 ng/ml) for 4 h and total RNA isolated was subjected to quantitative real-time PCR analysis for OIP-1 mRNA expression. B: IL-12 stimulation of OIP-1 and IFN-γ expression in human bone marrow cells. Bone marrow mononuclear cells were stimulated with IL-12 (10 ng/ml) for indicated time period (0–6 h) and total RNA isolated was subjected to real-time RT-PCR analysis for OIP-1 and IFN-γ mRNA expression. C: IL-12 stimulation of OIP-1 expression in human bone marrow cells cultured in the presence of anti-IFN-γ neutralizing antibody. Cells stimulated with IL-12 (10 ng/ml) for 4 h with/without IFN-γ antibody (50 U/ml) and total RNA isolated was subjected to quantitative real-time PCR analysis for OIP-1 expression. Relative levels of OIP-1 and IFN-γ gene expression were normalized with respect to the levels of GAPDH amplification. Values expressed as mean ±SE for three independent experiments. *P <0.05 level, #P <0.05 versus control and §P <0.05 versus IL-12 treated group.