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(A) Real time PCR for IGFBP3 in hTCEpi cells following incubation in 3.31 μM TSA for up to 30 hours and a non-treated control. There was a significant increase in IGFBP3 mRNA at 24 and 30 hours (n=3, mean ± standard deviation of 3 repeated experiments, One-way ANOVA, P<0.001). B: Conditioned media from hTCEpi cells treated with/without rhEGF (+/- E) and TSA (+/- T) for 48 hours. Treatment with TSA increased IGFBP3 secretion in conditioned media.
