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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Exp Eye Res. 2007 Jun 29;85(4):492–501. doi: 10.1016/j.exer.2007.06.015

Figure 6.

Figure 6

(A) hTCEpi cells grown on plastic and incubated with 20 μg of rhIGFBP3 demonstrated a corresponding increase in IGFBP3 protein levels. B-actin was used as a loading control. P: IGFBP3 plasmid expressed in hTCEpi cells as a positive control; R: 20 μg of recombinant human protein loaded into lane 2 as a positive control; 0 – 48 represent incubation times in hours. (B) Quantitative analysis of IGFBP3 as a function of incubation time. The addition of rhIGFBP3 resulted in an increase in cellular associated IGFBP3 compared to the control (n=3, mean ± standard deviation of 3 repeated experiments, One-way ANOVA, P<0.001).