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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Exp Eye Res. 2007 Jun 29;85(4):492–501. doi: 10.1016/j.exer.2007.06.015

Figure 7.

Figure 7

(A) hTCEpi cells grown on glass-bottom tissue culture dishes triple-labeled with Calcein-AM (live cells, green), Annexin V (early apoptotic cells, blue), and PI (dead cells, red) following incubation in rhIGFBP3. Membrane blebbing was seen in occasional cells at 24 hours. 20× magnification, scale: 44 μm. (B) hTCEpi cells grown on glass-bottom tissue culture dishes triple-labeled with Calcein-AM (live cells, green), Annexin V (early apoptotic cells, blue), and PI (dead cells, red) following incubation in TSA. Significant apoptosis was visible at 24 hours. 20× magnification, scale: 75 μm.