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The effect of capillary temperature and denaturants on resolution of two compression regions (I and II) in the M13mp18 sequence from base 386 to 423. (A) 25°C, 3.5 M urea, 30% formamide; (B) 50°C, 7 M urea; (C) 50°C, 3.5 M urea, 30 % formamide. The sieving matrix was 3% w/v LPA in 50 mM Tris-TAPS. With permission from [30].
