Table 1. IL-1β-dependent regulation of COX-2/PGE2.
HOC, TSU, and Tu686 HNSCC cells were stimulated with IL-1β (100 and 200 U/ml) for 18 hours. PGE2 concentration was then measured by EIA. Upon treatment with IL-1β, significant increases in PGE2 levels were noted Mean values are reported. All measurements were made in triplicate and repeated in at least 3 separate experiments.
| PGE2 levels (pg/ml) |
|||
|---|---|---|---|
| Cell Line | Control | IL-1β 100 U/ml | IL-1β 200 U/ml |
| HOC | 0 | 366 ± 13a | 440 ± 11a,b |
| TSU | 70 ± 3.5 | 303 ± 10a | 413 ± 9.6a,b |
| Tu686 | 8 ± 0.6 | 15 ± 1a | 35 ± 1.9a,b |
a
p<0.001 vs. Control
b
p<0.01 vs. 100U/ml