. Author manuscript; available in PMC: 2010 Sep 27.

Published in final edited form as: Transplantation. 2009 Sep 27;88(6):753–756. doi: 10.1097/TP.0b013e3181b443ae

Table 2.

PROCESSING PARAMETERS, ISLET CHARACTERISTICS, AND CLINICAL OUTCOMES OF ISLET ISOLATIONS USING THE SERVA BLEND

Processing Parameter	Mean ± SD (n=14)
Collagenase Dose (U/Pancreas)	1747 ± 212
Neutral Protease Dose (U/Pancreas)	244 ± 21
Digestion Time (minutes)	16.0 ± 2.0
Trimmed Pancreas Weight (g)	102 ± 18
Pancreas Digestion (%)	70 ± 17
Islet Characteristic	Mean ± SD (n=14)
Purified IEQ/g Trimmed Pancreas (IEQ)	5,862 ± 1,843
Cultured IEQ/g Trimmed Pancreas (IEQ)	5,458 ± 1,455
Post-Culture Recovery (%)	95 ± 15
Total IEQ at Transplant (IEQ)	544,745 ± 114,862
Islet Viability (%)	98.2 ± 2.3
Glucose Stimulation Index (μLU/ml)	3.15 ± 2.6
Clinical Outcomes	n
Pts with C-peptide at 1 month after Transplant	10/10^*
Patients Insulin Independent ≥ 30 days	9/10^*
Duration of Independence (months)	>24, >24, >21, >18, >13, >3, >6, >3, 4

6 patients received single islet transplants and 4 patients received 2 islet transplants. All patients produced C-peptide after their first transplant.