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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Neurobiol Aging. 2008 Apr 2;30(12):1962–1974. doi: 10.1016/j.neurobiolaging.2008.02.011

Figure 1.

Figure 1

The 3′ 30 bases of 52sce RNA are sufficient for protein-RNA complex formation, which is blocked by the 5′ 22 bases. (A) K562 whole cell lysate was incubated with radiolabelled 52sce, then treated with increasing amounts of RNAse T1. (B) K562 whole cell lysate was incubated with radiolabelled 52sce, then treated with indicated amounts of RNAse V1 or RNAse T1. (C) K562 whole cell lysate was incubated with radiolabelled full-length (FL) 52sce oligoribonucleotide, a truncated 52sce with 22 bases deleted from the 5′ end (Δ22), or an oligoribonucleotide containing the 3′ 30 bases of 52sce and the 22 bases immediately downstream of 52sce in the APP 3′ UTR (sh52sce). (D) FL, Δ 22 and sh52sce sequences are given. Numbering reflect APP751 cDNA sequence (accession #X06989).