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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Neurobiol Aging. 2008 Apr 2;30(12):1962–1974. doi: 10.1016/j.neurobiolaging.2008.02.011

Figure 2.

Figure 2

Purification and identification of 52sce-binding proteins. K562 whole cell lysate was incubated with biotinylated oligoribonucleotide (sh52sce; APP751 mRNA bases 2403 – 2454) tethered to a strepavidin affinity column matrix. The column was eluted with a 10-fold excess of free sh52sce, and column fractions were run out on a Northwestern blot probed with radiolabelled sh52sce. Active bands in the eluate (bands 1,2, and 3) were excised from an identically-ran SDS-PAGE gel and identified through Mass Spectroscopy. I: K562 total cell lysate; II: K562 total cell lysate pre-cleared with 52sce RNA; III: Affinity Column flow-through. IV: First eluate (with free sh52sce RNA); V: Second eluate (with SDS denaturing buffer).