Figure 3.
Purified 52sce-binding proteins complex with APP mRNA sequences in electrophoretic mobility shift assay. K562 total cell lysate was incubated with radiolabelled sh52sce RNA or with RNA corresponding to the first 250 bases of APP751 3′UTR (bases 2381 – 2630). Protein-RNA complexes were excised from native gels, and gel fragments electrophoresed into SDS-PAG followed by transfer and and immunoblot against indicated targets. Control: K562 total cell lysate.