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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Neurobiol Aging. 2008 Apr 2;30(12):1962–1974. doi: 10.1016/j.neurobiolaging.2008.02.011

Figure 5.

Figure 5

52sce-binding proteins co-immunoprecipitate with APP mRNA from a human neuroblastoma cell line. (A) The indicated proteins were immunoprecipitated from SH-SY5Y cytosol fraction (“Input”) with a specific antibody (“IP”) or with pre-immune mouse IgG (“(−)”). (B) mRNA from immunoprecipitates was reverse-transcribed with random primer and subjected to qPCR with APP-specific primers. The amount of APP signal, n = 3, was quantified and normalized to that seen in the pre-immune mouse IgG immunoprecipitate, ± S.E.M. (C) cDNA obtained from immunoprecipitates in a representative experiment in (B) was amplified with primers specific for the APP alternative splice site, and the PCR products were electrophoresed on a 1.5% agarose gel (inverted color scheme). Marker: pGEM DNA size markers (Promega); Input: SH-SY5Y cytosol; RT(−): RT without template; PCR(−): PCR without template. (D) Same set of immunoprecipitates as in (C) was amplified with primers specific for S26, and the PCR products were electrophoresed on a 1.5% agarose gel (inverted color scheme). All lanes are the same as in (C).