Base stacking orientations of the NarI, NarIIQ1, NarIIQ2, and NarIIQ3 (63) duplexes. (A) Unmodified duplex detailing base stacking corresponding to the modified duplexes. (B) NarIIQ1, NarIIQ2, and NarIIQ3 (63) duplexes detailing base stacking between the base pair at the modified position and its 5′ neighboring base pair, with the 5′ neighboring base pair aligned as in the unmodified duplex.