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. Author manuscript; available in PMC: 2009 Nov 25.

Published in final edited form as: Chembiochem. 2008 Jan 4;9(1):93–102. doi: 10.1002/cbic.200700251

Thermodynamic denaturation profiles of the HIV-1 frameshift-site RNA with and without GNB at varying concentrations of urea. UV absorbance at 260 nm is plotted versus temperature for the frameshift-site stem-loop RNA (2 µm) in the absence of GNB with urea at a concentration of 2 m (▲) or 5 m (). The sample of the GNB-bound RNA contained RNA (2 µm) in the presence of GNB (2.5 µm) with urea at a concentration of 2m (●) or 5 m (). The RNA–GNB complex was formed at high concentration (1.2 mm RNA+1.5 mm GNB) and diluted immediately prior to analysis. The low-temperature baseline absorbance was normalized to 1 for all samples.

Inline graphic — Thermodynamic denaturation profiles of the HIV-1 frameshift-site RNA with and without GNB at varying concentrations of urea. UV absorbance at 260 nm is plotted versus temperature for the frameshift-site stem-loop RNA (2 µm) in the absence of GNB with urea at a concentration of 2 m (▲) or 5 m (). The sample of the GNB-bound RNA contained RNA (2 µm) in the presence of GNB (2.5 µm) with urea at a concentration of 2m (●) or 5 m (). The RNA–GNB complex was formed at high concentration (1.2 mm RNA+1.5 mm GNB) and diluted immediately prior to analysis. The low-temperature baseline absorbance was normalized to 1 for all samples.