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. 1998 May 26;95(11):6492–6497. doi: 10.1073/pnas.95.11.6492

Figure 3.

Figure 3

Immunohistochemical and Western blot analyses of SMN in human CNS tissues. (A) Pyramidal neuron in the neocortex shows strong SMN immunolabeling of cytoplasm and nuclear gems. (Bar = 5 μm.) (B) Preadsorption of abSMN with 100-fold excess of peptide abolishes staining. (Bar = 50 μm.) (C) Low-magnification view of anterior horn cells within ventral gray matter of adult lumbar spinal cord. The cell bodies and proximal dendrites of α-motor neurons are intensely immunoreactive. (Bar = 230 μm.) (D) High-power view of adult anterior horn cell. (Bar = 20 μm.) (E) SMN labeling of nucleoli in cerebellar Purkinje cells often appeared as a solid ring of immunoreactivity circumscribing the outer surface of the nucleolus. (Bar = 10 μm.) (F) Western blot analysis of low-speed pellet material prepared from homogenates of human cerebellar autopsy tissue. After transfer to nitrocellulose, tissue fractions (20 μg total protein) were probed with either abSMN (i) or chicken antibody C3 (ii). Lanes: 1, autopsy case 96017; 2, autopsy case 97029; 3, autopsy case 97051.