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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Clin Cancer Res. 2009 Oct 20;15(21):6511–6518. doi: 10.1158/1078-0432.CCR-09-0565

Figure 1. Analysis of purified native mesothelin by gel electrophoresis and Western blot.

Figure 1

A. Soluble mesothelin purified from culture supernatant of a mesothelioma cell line or urine from ovarian and pancreatic cancer patients(19) was run on a 4–12% SDS gel (lane 2) and stained by SYPRO-Ruby. Lane 1 was loaded with molecular weight markers. B. Two duplicate lanes were used for further development by Western blot using the anti-human mesothelin mAb, K1 in lane 1 or serum from a patient with pancreatic adenocarcinoma negative for anti-mesothelin antibody (lane 2).