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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochem Pharmacol. 2009 Mar 27;78(1):33–44. doi: 10.1016/j.bcp.2009.03.017

Fig. 1.

Fig. 1

Induction of apoptosis of Jurkat cells by 2-ME2. (A) Dose-dependent induction of apoptosis of Jurkat cells. 1.5 × 106 cells were treated with increasing concentrations of 2-ME2 for 24 and 48 h as indicated or ethanol (Et) as control and low molecular weight DNA was extracted and analyzed by agarose gel electrophoresis. (B) Time-dependent apoptosis of Jurkat cells. 1.5 × 106 cells were treated with 0.5 and 1.0 μM of 2-ME2 or Et for different periods of time as indicated and low molecular weight DNA was extracted and analyzed by agarose gel electrophoresis. (C) Analysis of apoptosis of asynchronous cultures of Jurkat cells treated with 2-ME2 as determined by flow cytometry. Cells treated with 0.5 and 1.0 μM of 2-ME2 or Et for 12 and 24 h were subjected to flow cytometric analysis as described under materials and methods. Results were reproduced in two independent experiments.