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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochem Pharmacol. 2009 Mar 27;78(1):33–44. doi: 10.1016/j.bcp.2009.03.017

Fig. 2.

Fig. 2

Bcl-2 suppressed Jurkat cell apoptosis induced by 2-ME2 through the mitochondrial pathway. (A) Expression of Bcl-2 in retrovirally-transduced Jurkat cells. Total proteins extracted from Jurkat, Jurkat Puro and Jurkat Bcl-2 cells were analyzed by immunobloting for the expression of Bcl-2. (B) DNA fragmentation analysis of uninfected and retrovirus-infected Jurkat cells. DNA isolated from untreated and ethanol- or 2-ME2-treated Jurkat, Jurkat Puro and Jurkat Bcl-2 cells was analyzed by agarose gel electrophoresis. (C) Expression of Bcl-2 and Bak in control and Puro- or Bcl-2-expressing cells following treatment with 2-ME2. Total proteins isolated were analyzed by immunoblotting for the expression of Bcl-2- or Bak. In the Bcl-2 blot, the upper slower migrating band represents the phosphorylated form of Bcl-2. (D) Expression of total and phospho-JNK (Thr183/Tyr185) in control and Puro- or Bcl-2-expressing cells following treatment with 2-ME2. (E) Expression of caspases and PARP-1. Total proteins isolated as in (C) were analyzed for the expression of caspase-9, caspase-3 and PARP-1 or β-actin. All experiments were repeated 2-3 times with comparable results.