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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochem Pharmacol. 2009 Mar 27;78(1):33–44. doi: 10.1016/j.bcp.2009.03.017

Fig. 3.

Fig. 3

Bcl-2-mediated apoptotic block is linked to G1/S cell cycle arrest following treatment with 2-ME2. (A) Growth curves of uninfected and retrovirus-infected Jurkat cells in the presence or absence of 2-ME2, over a period of nine days. Each point represents the average of two wells in three independent experiments. (B) Flow cytometric analysis of asynchronous cultures of Puro- and Bcl-2-expressing Jurkat cells treated with 2-ME2. Jurkat Puro and Jurkat Bcl-2 cells treated with 2-ME2 or ethanol (Et) for 12 and 24 h were subjected to flow cytometric analysis. (C) Evaluation of apoptosis by annexin V/PI staining. Jurkat Puro and Jurkat Bcl-2 cells were treated with ethanol or 1.0 μM 2-ME2 for 24 h and subjected to annexin V/PI staining. Black bars represent viable cells and grey bars represent apoptotic cells.