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Expression of cell cycle regulatory proteins in control and Puro- or Bcl-2-expressing Jurkat cells following treatment with 2-ME2. Cells were left untreated or treated with ethanol, or 0.5 μM and 1.0 μM 2-ME2 for 24 h. Total proteins isolated from untreated and ethanol- or 2-ME2-treated cells were analyzed by immunoblotting for the expression of cyclin D3, cyclin E, E2F1, pRb p16INK4A, p21Cip1/Waf1 and p27Kip1 or β-actin. Experiments were repeated 2-3 times with comparable results.
