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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochem Pharmacol. 2009 Mar 27;78(1):33–44. doi: 10.1016/j.bcp.2009.03.017

Fig. 5.

Fig. 5

Subcellular expression of Bcl-2 and p27Kip1 in Puro- or Bcl-2-expressing Jurkat cells following 2-ME2 treatment. Cytoplasmic and nuclear fractions (A) or Nuclei (B) were isolated from Jurkat Puro and Jurkat Bcl-2 cells cultured in the absence or presence of ethanol or 2-ME2. The fractions were analyzed by immunoblotting for the expression of Bcl-2 or p27Kip1. Equal loading and verification of extracts was detected using an anti-Lamin B or anti-β-actin. No actin was detected in nuclear extracts or from isolated nuclei. All experiments were repeated multple times with similar results.