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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochem Pharmacol. 2009 Mar 27;78(1):33–44. doi: 10.1016/j.bcp.2009.03.017

Fig. 6.

Fig. 6

Expression of NF-κB and Pim-2 in Puro- and Bcl-2-expressing Jurkat cells following 2-ME2 treatment. Total proteins (A), Cytoplasmic and nuclear fractions (B) or Nuclei (C) isolated from untreated, ethanol- or 2-ME2-treated Jurkat Puro and Jurkat Bcl-2 cells were subjected to immunoblotting for the expression of the indicated proteins. (D) Expression of Pim-2, a NF-κB target protein. Total proteins isolated from untreated and ethanol- or 2-ME2-treated Jurkat Puro and Jurkat Bcl-2 cells were analyzed by immunoblotting for the expression of Pim-2. Equal loading and verification of extracts was detected using a rabbit polyclonal anti-Lamin B or a mouse monoclonal anti-β-actin. Experiments were repeated twice producing similar results.