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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochem Pharmacol. 2009 Mar 27;78(1):33–44. doi: 10.1016/j.bcp.2009.03.017

Fig. 7.

Fig. 7

Suppression of NF-κB signaling pathway in Jurkat cells. Jurkat and Jurkat Bcl-2 cells were infected with recombinant retrovirus carrying α IκBαSR to generate Jurkat IκBαSR and Jurkat Bcl-2/IκBαSR cells. (A) Total lysates from all the different cell types were analyzed by immunoblotting for the expression of IκBα or β-actin. (B) Total lysates from all the different cell types were analyzed by western immunoblot for the expression of p27Kip1 and Pim-2 or β-actin. (C) DNA was isolated from untreated, ethanol- or 2-ME2-treated cells as indicated and analyzed by agarose gel electrophoresis. Similar results were obtained in another independent experiment (data not shown.