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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochem Pharmacol. 2009 Mar 27;78(1):33–44. doi: 10.1016/j.bcp.2009.03.017

Fig. 8.

Fig. 8

Reduction of p27Kip1 levels sensitized Jurkat and Jurkat Bcl-2 cells to apoptosis. Jurkat and Jurkat Bcl-2 cells were infected with recombinant retrovirus bearing shRNA to p27Kip1 or a control vector to generate Jurkat Vec Jurkat p27KD, Jurkat Bcl-2/Vec and Jurkat Bcl-2/p27KD cells. (A) Total lysates from all the different cell types were analyzed by immunoblotting for the expression of p27Kip1 or β-actin. (B) and (C) Low molecular weight DNA was isolated from untreated, ethanol- or 2-ME2-treated Jurkat/Vec and Jurkat p27KD cells (B) or Jurkat Bcl-2/Vec and Jurkat Bcl-2/p27KD cells (C), as indicated and analyzed by agarose gel electrophoresis. Vec, vector pSR-Puro. All experiments were repeated twice with comparable results.