Table I.
Evaluation of the requirement for major Th cytokines for aerosolized Ag-induced recruitment of pulmonary MCp in sensitized BALB/c mice
| Cell and/or Mediator Tested | Deficient strain or mAba | Mean % of Control Lung MCp/106 MNCb (variance) | Mean % of Control Lung MCp/Mouseb (variance) | No. of Expt.c (no. of mice) |
|---|---|---|---|---|
| Null mice | ||||
| Th2/IL-4 | IL-4−/− | 142 (19) | 200 (78) | 5 (7) |
| IL-4Rα−/− | 63 (15) | 118 (18) | 4 (6) | |
| STAT-6−/− | 95 (36) | 99 (21) | 3 (4) | |
| Th1/IFN-γ | IFN-γ−/− | 170 (140) | 90 (50) | 2 (2) |
| Th1/17-IL-12/23 | IL-12p40−− | 71 | 137 | 1 (2) |
| Ab blockade | ||||
| IL-3 | Anti-IL-3 | 148 | 112 | 1 (2) |
| IL-4 | Anti-IL-4 | 64 (3) | 69 (10) | 2 (2) |
| IL-5 | Anti-IL-5 | 218 (27) | 284 (83) | 2 (2) |
| IL-6 | Anti-IL-6 | 126 (11) | 165 (48) | 1 (3) |
| IL-10 | Anti-IL-10 | 98 | 341 | 1 (1) |
| IL-13 | Anti-IL-13 | 106 (23) | 98 (29) | 2 (4) |
| IFN-γ | Anti-IFN-γ | 138 (30) | 155 (100) | 2 (2) |
| IL-12p40 | Anti-IL-12p40 | 78 | 93 | 1 (2) |
| Anti-IL-12Rβ1 | 86 | 88 | 1 (2) | |
| IL-17A | Anti-IL-17A | 111 (29) | 129 (42) | 1 (3) |
| Treg | Anti-CD25 | 146 (46) | 270 (16) | 2 (4) |
All mice listed were on a BALB/c background and were sensitized and challenged. The increment in total lung MCp over baseline was >10-fold in the different experiments. For mAb inhibition, mice were injected with 100 μg of the indicated mAb before each challenge. For Treg depletion, mice were injected with 1 mg of mAb on days −1, 6, and 16.
The values represent the influx of MCp as measured by lung MCp concentration (MCp/106 MNC) or by total lung MCp per mouse as a percentage of the values obtained from control mice treated in parallel. The controls were WT mice for deficient strains and WT mice treated with isotype-matched IgG for WT mice receiving blocking mAb. The values are the mean with the variance in parentheses: SE for three or more experiments or 1/2 range for two experiments.
Number of experiments and in parentheses the number of mice evaluated.