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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Methods Enzymol. 2009;457:349–372. doi: 10.1016/S0076-6879(09)05020-4

Figure 3. Testing the integrity of the outer mitochondrial membrane.

Figure 3

Oxygen concentration (grey line) and respiration rate (black line) measured using a polarographic oxygen electrode. Respiration is stimulated by the addition of substrates glutamate and malate (GM) and ADP to stimulate state 3 respiration. 10 μM cytochrome c is added to assess outer membrane integrity. An increment in respiration with exogenous cytochrome c is apparent if the outer mitochondrial membrane is damaged (panel A). Panel B illustrates a preparation where membrane integrity is high.