Fig. 4.
SDS-PAGE of BglA protein extracted from E coli BL21 (DE3) and undergoing various steps of purifications. Lane a: bacteria cell extract; Lane b: molecular mass standards; Lane c: (NH4)2SO4 precipitate (55 to 60% saturation); Lane d: pooled DEAE Sepharose fractions; Lane e: pooled Sephacryl fractions. The narrow band indicates BglA protein.