Figure 3.

SCN molecular rhythms persist in culture without CLOCK. Bioluminescence rhythms from cultures of the SCN of wild-type mice expressing mPeriod2::Luciferase (Clock^+/+;mPer2^Luc, top panel) and CLOCK-deficient mice expressing the fusion protein (Clock^−/−;mPer2^Luc, bottom panel). The data shown (n = 2, wild-type SCN; n = 2, CLOCK-deficient SCN) were normalized by the average luciferase activity over the duration of the experiment and 24-h trends were removed. The raw data from these and additional experiments are shown in Supplementary Figure 1.