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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Biochemistry. 2009 Oct 13;48(40):9627–9640. doi: 10.1021/bi901391g

Figure 6. Phosphoethanolamine-modified lipid IVA in CMR200, a PmrA-constitutive kdtA deletion mutant.

Figure 6

Cells were grown at 30 °C and lipids were extracted as in Fig. 4. Panel A. ESI/MS in the negative ion mode of the major lipid species in the control strain MST100(pWMsbA). Panel B. Accumulation of the pEtN-modified lipids IIIA and IIIB (11, 12) in CMR200, indicated by the red numbers. The accumulation of lipids IIIA ([M-2H]2− at m/z 762.931) and IIIB ([M-2H]2− at m/z 882.052) was confirmed by TLC (insets).