Fig. 8. Processing of the LEE4 transcript at the end of sepL diminishes SepL protein level.

Bacteria were grown in DMEM 0.2 % glucose and cellular proteins from bacterial pellets were subjected to SDS-PAGE and Western blotting with the indicated antisera raised against SepL (A) or EspA(B). The amount of protein loaded in the gel was standardized by OD₆₀₀ readings for strains UMD878 (EPECsepL) and the complemented strain UMD878 (pCO24) (EPECsepL(pSepL)). For the other samples, protein concentration was measured and adjusted before loading the gel. After immunoblot, the membranes were stained with Brilliant Blue R (Sigma) to show total protein (right panels in (A) and (B)).