Table 1.
Primers sequences used for real-time RT-PCR.
| Gene | Primers |
|---|---|
| ACTB | Fwd: 5'-TGA GCG CAA GTA CTC TGT GTG GAT-3' |
| Rvs: 5'-AGA AGC ATT TGC GGT GCA CGA T-3' | |
| IL1B | Fwd: 5'-AGG ATT GCT TCC AAG CCC TTG ACT-3' |
| Rvs: 5'-ACA GCT TCT CCA CAG CCA CAA TGA-3' | |
| IL6 | Fwd: 5'-CAA GAG ACT TCC AGC CAG TTG C-3' |
| Rvs: 5'-TGT TGT GGG TGG TAT CCT CTG TG-3' | |
| TGFB1 | Fwd: 5'-TGG CGT TAC CTT GGT AAC C-3' |
| Rvs: 5'-GGT GTT GAG CCC TTT CCA G-3' | |
| COX2 | Fwd: 5'- TCC AGT ATC AGA ACC GCA TTG CCT-3' |
| Rvs: 5'-AGC AAG TCC GTG TTC AAG GAG GAT-3 | |
| COL1A1 | Fwd: 5'-AGG CAT AAA GGG TCA TCG TGG CTT-3' |
| Rvs: 5'-AGT CCA TCT TTG CCA GGA GAA CCA-3' | |
| COL3A1 | Fwd: 5'-ATG AGC TTT GTG CAA TGT GGG ACC-3' |
| Rvs: 5'-ACT GAC CAA GGT AGT TGC ATC CCA-3' | |
| MMP8 | Fwd: 5'-TGG ACA CAC ACT AAC CTG ACC TAC-3' |
| Rvs: 5'-TCT CCC TCT AAG GTC TCA GTG AAG-3' | |
| MMP13 | Fwd: 5'-AAA GAA CAT GGT GAC TTC TAC C-3' |
| Rvs: 5'-ACT GGA TTC CTT GAA CGT C-3' |
Note: ACTB, β-actin; TGFB1, transforming growth factor-β1; COX2, cyclooxygenase-2; IL1B, interleukin-1β; IL6, interleukin-6; COL1A1, collagen type 1 α1; COL3A1, collagen type 3 α1; MMP8, matrix metalloproteinase 8; MMP13, matrix metalloproteinase 13. Standard gene symbols were obtained from the HUGO Gene Nomenclature Committee (http://www.genenames.org).