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Average intracellular concentrations of FD-2000 as a function of the fractional area of pores in the permeabilized membrane (α) for different pore opening periods (T): (a) T = 0.1 msec; (b) T = 1 msec; and (c) T = 10 msec. The average concentrations were normalized by the concentration in pulsing medium (C0), which depended on average pore diameter ranging from 55 to 500 nm in the simulation.
