Table 1.

After Three Rounds of Selection on Medium Containing Antibiotics, Transgenic Plants Were Analyzed Via Direct PCR.

	ΔftsZ1-1	ΔftsZ1-2	ΔftsZ3	ΔftsZ2-2	ΔftsZ1-1/1–2	ΔftsZ2-1/2–2
Direct PCR	180	132	238	34	46	192
Correct 5’ and 3’ integration	22*	19*	9	5	11*	2
Absence of WT transcript	4	7	9	5	5	2

All plants with correct 5’ and 3’ integration of the knockout construct into the respective locus were further tested for absence of the ftsZ transcript via RT–PCR. * RNA for subsequent RT–PCR analysis was extracted from only a subset of the plants.