Figure 2.
Detection of PRK in Elysia chlorotica.
(A) PRK Western blot using a tobacco PRK antibody and the alkaline phosphatase detection system. Extracts were loaded on an equal chlorophyll basis as follows: E-kp, E. chlorotica kleptoplasts (from animals starved nine months); V-cp, V. litorea plastids; S-cp, spinach plastids; E-tp, E. chlorotica total proteins (from animals starved for 3 months); V-tp, V. litorea total proteins.
(B) Western blot and fluorogram of immunoprecipitated PRK from 5-month-starved sea slugs, following labeling with [35S]methionine/cysteine for 6 h and separation by SDS–PAGE.