PCR Amplification of prk from V. litorea and E. chlorotica.
(A) PCR amplification of prk from algal and sea slug DNA using primers PRK5F and PRK5R. Lane 1, 1 kb Plus DNA ladder (Invitrogen); lane 2, no DNA (negative control); lane 3, pVA1prk construct (pGEM-T Easy vector with the V. litorea prk cDNA as insert; positive control); lane 4, pVA2prk construct (pGEM-T Easy vector with genomic V. litorea prk as insert); and lane 5, E. chlorotica DNA as template.
(B) PCR amplification of the prk gene from sea slug egg DNA using primers PRK5F and PRK5R. Lane 1, 1 kb plus ladder; lane 2, no DNA (negative control); lane 3, sea slug egg DNA. The arrows in both figures point to prkY (421 bp) and prkX (269 bp) fragments.