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. 2009 Oct 30;2(6):1384–1396. doi: 10.1093/mp/ssp085

Figure 6.

Figure 6.

PCR Amplification of prk from V. litorea and E. chlorotica.

(A) PCR amplification of prk from algal and sea slug DNA using primers PRK5F and PRK5R. Lane 1, 1 kb Plus DNA ladder (Invitrogen); lane 2, no DNA (negative control); lane 3, pVA1prk construct (pGEM-T Easy vector with the V. litorea prk cDNA as insert; positive control); lane 4, pVA2prk construct (pGEM-T Easy vector with genomic V. litorea prk as insert); and lane 5, E. chlorotica DNA as template.

(B) PCR amplification of the prk gene from sea slug egg DNA using primers PRK5F and PRK5R. Lane 1, 1 kb plus ladder; lane 2, no DNA (negative control); lane 3, sea slug egg DNA. The arrows in both figures point to prkY (421 bp) and prkX (269 bp) fragments.