Figure 3.
Effect of testosterone withdrawal on growth of LNCaP/Luc tumour xenografts in nude mice. (A) Relative tumour volume (RTV) of LNCaP/Luc tumours over 36 days. Each point represents the mean±s.e.m. of six to eight observations. Treatment groups assigned on day 20. **P<0.01, *P<0.05 (Mann–Whitney analysis). (B) Light emission from LNCaP/Luc tumours normalized to tumour volume (mm3) at day 36. (C) Bioluminescent imaging of luciferase activity in testosterone-treated and vehicle-treated mice harbouring LNCaP/Luc tumours at day 36, following injection with d-luciferin. (D) Immunohistochemical staining of Ki67 (upper panel) and phospho-Histone H3 (lower panel) from sections of LNCaP/Luc tumour xenografts from mice treated with testosterone or vehicle. Scale bar, 25 μm. Counting results from five separate tumours is shown on the right.