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. 2009 Oct;7(4):305–312. doi: 10.2450/2009.0081-08

Figure 2.

Figure 2

Expression of the recombinant FVII by stable clones of CHO. RNA was extracted from the CHO cells transfected with the pDEST26-FVII construct and the CHO cells transfected with the empty vector. cDNA was synthesised and RT-PCR was performed. CHO cells transfected with the construct expressed FVII mRNA, an approximately 1400 bp length fragment (Lane 2), while no expression was observed in CHO cells transfected with empty vector (Lane 1). The lower figure shows the expression of β-actin in both stable clones of CHO, i.e. transfected with the pDEST26-FVII or empty vector. M: 100 bp ladder marker.