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. 2009 Oct;7(4):305–312. doi: 10.2450/2009.0081-08

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Detection of recombinant FVII by ELISA. Cell culture medium and lysate of the stable clones were evaluated. The expression of recombinant FVII was confirmed by ELISA. His-tagged FVII was detected both in cell culture medium and cell lysate. Two stable clones (Clone 1 and 2), CHO transfected with pcDNA 3.1-FVII and empty vector are shown in the figure. No FVII expression was detected in the medium of CHO cells transfected with empty vector (pcDEST26 3.1). Data are presented as mean ± SD.