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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: JALA Charlottesv Va. 2009 Dec 1;14(6):331–340. doi: 10.1016/j.jala.2009.06.003

Figure 3.

Figure 3

The importance of controlling the washing conditions during phage selection. a) In the first round of selection, the percentage of recovered phage as a function of washing time decays non-linearly, as non-specifically bound and weak binding phage are removed. When modeled as a first order exponential (dashed line), the dissociation rate constant was kd1 = 1.0±0.1∙10−3 s−1. (Inset) The canonical target-binding peptide motif (HPQ) was not found in clones isolated after the first round. b) In the second round, the percentage of bound phage also showed an exponential decay as stringency (washing time) increased, with a remarkably similar dissociation rate constant of kd2 = 1.07±0.04∙10−3 s−1. (Inset) The percentage of clones with the HPQ motif increased monotonically as a function of washing time; after 120 minutes of washing, 8 out of 9 clones contained this motif. Figure reprinted from Liu et al.36 with permission of the authors.