. Author manuscript; available in PMC: 2010 Nov 15.

Published in final edited form as: J Immunol. 2009 Oct 30;183(10):6500–6512. doi: 10.4049/jimmunol.0901521

Table I.

Properties of the MARCH1 mutants

MARCH1 constructs:	WT	RING mutant (W104A)	ΔN1-40	ΔN1-66	ΔN1-121	ΔN120-141	ΔC257-279	ΔC229-279	Y118F	L215S, Y222F
Steady-state levels	+	+	++	+++	++++	−	+	+	+	+
Function^a (CD86, MHC II)	+++	−	+++	+++	−	−	++	−	+++	+/−
Cellular localization^b	LAMP-1⁺, Furin ⁺⁺⁺	N/A^c	N/A	Furin⁺, LAMP-1⁺ & other	Furin⁺, LAMP-1⁺ & other	N/A	N/A	LAMP-1⁺⁺ Furin⁺⁺⁺	N/A	N/A

Function – determined by analyzing the surface expression of either CD86 or MHC class II in the presence of each MARCH1 construct using flow cytometry (“+” indicates a functional MARCH1 construct; “−” represents a non-functional MARCH1 construct; see Figure 5)

Localization – determined by co-staining with HA antibody against MARCH1 and either Derlin-1, LAMP-1, Furin, or EEA-1 (see Figure 6 and Figure S2 for complete immunofluorescence analysis)

Not available