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Phosphorylation of Retinoblastoma protein (ser 608) following IGF-1 activation. SV and IMA SMCs were stimulated with IGF-1 (100 ng/ml) over a time course of 0–24 hrs. Phosphorylation of retinoblastoma protein was determined by Western blotting and densitometric analysis of five individual samples of SV and IMA cells is represented graphically. The phosphorylation of Rb is shown with relation to the restriction (R) point. (*P < 0.001; *P < 0.05)
