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Effect of PTEN gene silencing on the expression of cyclin E, phosphorylation of Rb protein, p21 and p27 in SV SMCs. The transfected cells were stimulated with IGF-1 (100 ng/ml). Expression levels of cyclin E (A) and pRb, p21 and p27 (B) were determined by Western blotting and the densitometric analysis of five individual samples of SV cells (C) is represented graphically. (*P < 0.01 compared with control group. #P < 0.01 compared with IGF-1 + empty vector).
