Skip to main content
. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: Mol Cell. 2009 Nov 13;36(3):500–511. doi: 10.1016/j.molcel.2009.10.021

Figure 4. Effect of GM1 on Mitochondrial Transmembrane Potential and Opening of the PTP.

Figure 4

(A) Live measurements of TMRM fluorescence in individual mitochondria of β-gal+/+, β-gal+/+ loaded with GM1, and β-gal−/− MEFs pulsed with histamine and depolarized with CCCP as indicated. Values are expressed as percentage of initial TMRM fluorescence and represent the mean ± SE of 20 independent measurements.

(B) Live measurements of TMRM fluorescence in individual mitochondria of β-gal−/−/CyP-D−/− MEFs compared to TMRM fluorescence in β-gal−/− and β-gal+/+ mitochondria. Values are expressed as percentage of initial TMRM fluorescence and represent the mean ± SE of 20 independent measurements.

(C) Restoration of the ΔΨm in β-gal+/+ loaded with GM1 and β-gal−/− MEFs treated with MBCD. Values are expressed as percentage of initial TMRM fluorescence and represent the mean ± SE of 20 independent measurements.

(D) Live measurements of mitochondrial calcein release in β-gal+/+, β-gal+/+ loaded with GM1, and β-gal−/− MEFs pulsed with CoCl2. Data are expressed as percentage of initial calcein fluorescence and represent the mean ± SE of at least 20 independent measurements.

(E) Live measurements of mitochondrial calcein release in β-gal−/−/CyP-D−/− MEFs pulsed with CoCl2 and compared to the values obtained in β-gal−/− and β-gal+/+ mitochondria. Data are expressed as percentage of initial calcein fluorescence and represent the mean ± SE of at least 20 independent measurements.

(F) Prevention of PTP opening β-gal+/+ loaded with GM1 and β-gal−/− MEFs treated with MBCD. Data are expressed as percentage of initial calcein fluorescence and represent the mean ± SE of at least 20 independent measurements.