Figure 2. Agonist Peptides E1, T1, and OVAp Cause Deletion of CD8WTOT-1 Thymocytes in FTOC.
FTOC was performed with CD8WTOT-1 transgenic thymic lobes in the presence of media or 50 μM E1, T1, or OVA peptides. Thymocytes from cultured lobes were harvested after 7 days, counted, and analyzed for expression of CD4 and CD8 by flow cytometry.