Figure 5. CD8°OT-1 Thymocytes Selected by OVAp Variants Proliferate in Response to Antigen.

FTOC was performed with CD8°OT-1 transgenic fetuses as described in Figure 3. Thymic lobes were cultured for 7 days in the presence of media or 50 μM E1, T1, or OVA peptides. At the end of FTOC, cells were harvested and added to cultures at one quarter-lobe equivalent per well with irradiated TAP° splenocytes as antigen-presenting cells with or without 100 nM OVAp. Cultures were pulsed for 8 hr with [³H]thymidine after 36 hr of culture. Data are shown as the mean and standard deviation and are representative of three experiments.