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Flow migration chamber from the top (A) and the side views (B). Micrograph of an EI cell monolayer that was grown on the filter (C). EI cells provide a consistent cell substrate with a stable level of ICAM-1 expression. Circulating flow provides a model of a microvascular environment where a cell monolayer acts as a barrier that cells must migrate through in response to chemotactic stimulation from the bottom wells (D).
