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PLC inhibitor U73122 inhibits endothelial [Ca2+]i responses following contact melanoma cells. HUVEC were pre-incubated with 2, 5, 7 and 10 μM U73122 for 5 min prior to an assay. A complete elimination of melanoma cell-induced [Ca2+]i responses was observed at the concentration of 10 μM. Results are presented with mean ± SEM; n = 3~5.
