Skip to main content
. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Front Biosci. 2005 May 1;10:1597–1606. doi: 10.2741/1643

Figure 5.

Figure 5

Melanoma cells induce junction opening and disassembly of VE-cadherin homophilic binding. Endothelial cells were fixed and stained for VE-cadherin using VE-cadherin mAb. Panel (A) shows 100× fluorescence images of continuous VE-cadherin staining patterns without melanoma cell contact. Contact of melanoma to EC for 10 min did not induce junction opening in panel (B). Contrastively, disassembled junctions caused by melanoma cell contacts at 45 min are shown in panel (C). Asterisks indicate the sites where gaps formed. The results are representative of 3 experiments. For negative control, secondary antibody alone did not contribute to any background signal of nonspecific binding (data not shown).