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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Front Biosci. 2005 May 1;10:1597–1606. doi: 10.2741/1643

Figure 6.

Figure 6

PLC activity participates in melanoma cell-mediated junction regulation. (A) VE-cadherin staining patterns (100×) in response to melanoma cell contacts at 45 min. (B) HUVEC were pretreated with 10 μM U73122 for 5 min prior to melanoma cell addition. A majority of junctions remained continuous at 45 min in the 100× fluorescence image while only a small population of endothelial cells responded to melanoma cell contacts by opening of junctions. (C) Pretreatment with 20 μM Ly294002 did not reduce VE-cadherin disassembly (100×) at 45 min. Asterisks indicate the sites where gaps formed. The results are representative of 3 experiments.