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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Front Biosci. 2005 May 1;10:1597–1606. doi: 10.2741/1643

Figure 7.

Figure 7

Tyrosine phosphorylation of VE-cadherin was not altered upon melanoma cell contact. HUVEC lysates were immunoprecipitated with VE-cadherin Ab after 45 min with and without melanoma cell contacts. Precipitated proteins were analyzed by immunoblotting with an Ab against phosphotyrosine. The same blot was stripped and then reprobed with Ab to VE-cadherin. VE-cadheirn was not found to be tyrosine phosphorylated in both cases (+ and −). VEGF, an inducer of VE-cadherin tyrosine phosphorylation, used at the concentration of 50 ng/ml altered the phosphotyrosine after 30 min. Results are representative of 7 experiments.