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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Cardiovasc Res. 2008 Apr 30;79(2):313–321. doi: 10.1093/cvr/cvn108

Figure 2. Ethanol stimulates HUVEC growth and migration.

Figure 2

HUVEC were treated with growth media in the absence (control) or presence of ethanol (25 mM) as described in Methods. (a) Cell counts of parallel triplicate wells were made on a daily basis. (b) Proliferating cell nuclear antigen (PCNA) protein expression in control and ethanol treated (25 mM, 24 h) endothelial cells; representative Western blot (top), cumulative densitometric data, n=3 (bottom). (c) Bar graph showing increased migration (scratch wound assay) by ethanol treated HUVEC at 12 and 24 h compared to control cells. *p<0.05 vs respective control.